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DNA repair pathways underlie a common genetic mechanism modulating onset in polyglutamine diseases

Bettencourt, Conceicao, Hensman-Moss, Davina J., Flower, Michael, Wiethoff, Sarah, Brice, Alexis, Goizet, Cyril, Stevanin, Giovanni, Koutis, Georgios, Karadima, Georgia, Panos, Marios, Yescas-Gomez, Petra, Garcia-Velazquez, Lizbeth Esmeralda, Alonso-Vilatela, Maria Elisa, Lima, Manuela, Raposo, Mafalda, Traynor, Bryan, Sweeney, Mary, Wood, Nicholas, Guinti, Paola, Durr, Alexandra, Holmans, Peter, Houlden, Henry, Tabrizi, Sarah J. and Jones, Lesley 2016. DNA repair pathways underlie a common genetic mechanism modulating onset in polyglutamine diseases. Annals of Neurology 79 (6) , pp. 983-990. 10.1002/ana.24656

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Abstract

Objective: The polyglutamine diseases, including Huntington’s disease (HD) and multiple spinocerebellar ataxias (SCAs), are amongst the commonest hereditary neurodegenerative diseases. They are caused by expanded CAG tracts, encoding glutamine, in different genes. Longer CAG repeat tracts are associated with earlier ages at onset, but this does not account for all of the difference, and the existence of additional genetic modifying factors has been suggested in these diseases. A recent GWAS in HD found association between age at onset and genetic variants in DNA repair pathways and we therefore tested whether the modifying effects of variants in DNA repair genes have wider effects in the polyglutamine diseases. Methods: We assembled an independent cohort of 1462 subjects with HD and polyglutamine SCAs, and genotyped SNPs selected from the most significant hits in the HD study. Results: In the analysis of DNA repair genes as a group, we found the most significant association with age at onset when grouping all polyglutamine diseases (HD+SCAs, p=1.43x10-5). In individual SNP analysis, we found significant associations for rs3512 in FAN1 with HD+SCAs (p=1.52x10-5) and All SCAs (p=2.22x10-4) and rs1805323 in PMS2 with HD+SCAs (p=3.14x10-5), all in the same direction as in the HD GWAS. Interpretation: We show that DNA repair genes significantly modify the age at onset in HD and SCAs, suggesting a common pathogenic mechanism, which could operate through the observed somatic expansion of repeats that can be modulated by genetic manipulation of DNA repair in disease models. This offers novel therapeutic opportunities in multiple diseases.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Additional Information: The SPATAX Network Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0364-5134/ (accessed 21/06/2016)
Publisher: Wiley
ISSN: 0364-5134
Funders: MRC, Wellcome Trust
Date of First Compliant Deposit: 7 April 2016
Date of Acceptance: 31 March 2016
Last Modified: 15 Jun 2020 14:30
URI: http://orca.cf.ac.uk/id/eprint/88933

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