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Polypropylene sulfide nanoparticle p24 vaccine promotes dendritic cell-mediated specific immune responses against HIV-1

Caucheteux, Stephan M., Mitchell, John P., Ivory, Matthew O., Hirosue, Sachiko, Hakobyan, Svetlana, Dolton, Garry, Ladell, Kristin Ingrid, Miners, Kelly Louise, Price, David, Kan-Mitchell, June, Sewell, Andrew K., Nestle, Frank, Moris, Arnaud, Karoo, Richard O., Birchall, James Caradoc, Swartz, Melody A., Hubbel, Jeffrey A., Blanchet, Fabien P. and Piguet, Vincent 2016. Polypropylene sulfide nanoparticle p24 vaccine promotes dendritic cell-mediated specific immune responses against HIV-1. Journal of Investigative Dermatology 136 (6) , pp. 1172-1181. 10.1016/j.jid.2016.01.033

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Abstract

Delivery of vaccine formulations into the dermis using antigen-coated microneedle patches is a promising and safe approach because of efficient antigen delivery and safety. We evaluated an intradermal vaccine using HIV-1 p24 Gag peptide-conjugated polypropylene sulfide nanoparticles to induce immunity against HIV-1. This peptide-conjugated polypropylene sulfide nanoparticle formulation did not accelerate the maturation of blood- or skin-derived subsets of dendritic cells, either generated in vitro or purified ex vivo, despite efficient uptake in the absence of adjuvant. Moreover, dendritic cell-mediated capture of particulate antigen in this form induced potent HIV-1–specific CD4+ T-cell responses, as well as B-cell–mediated antibody production. Nanoparticle-based intradermal antigen delivery may therefore provide a new option in the global effort to develop an effective vaccine against HIV-1.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Pharmacy
Subjects: Q Science > QR Microbiology > QR180 Immunology
Uncontrolled Keywords: DC, dendritic cell; ID, intradermal; LC, Langerhans cell; LPS, lipopolysaccharide; MDDC, monocyte-derived dendritic cell; MDLC, monocyte-derived Langerhans cell; NP, nanoparticle; p24, HIV-1 capsid protein; p24NP, HIV-1 p24 Gag peptide-conjugated polypropylene sulfide nanoparticle; TNF, tumor necrosis factor
Publisher: Nature Publishing Group
ISSN: 0022-202X
Date of First Compliant Deposit: 11 April 2016
Date of Acceptance: 14 January 2016
Last Modified: 29 Jun 2019 09:44
URI: http://orca.cf.ac.uk/id/eprint/89071

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