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Glutathione transferase (GST) as a candidate molecular-based biomarker for soil toxin exposure in the earthworm Lumbricus rubellus

LaCourse, E. J., Hernandez-Viadel, M., Jefferies, J. R., Svendsen, C., Spurgeon, D. J., Barrett, J., Morgan, Andrew John, Kille, Peter and Brophy, P. M. 2009. Glutathione transferase (GST) as a candidate molecular-based biomarker for soil toxin exposure in the earthworm Lumbricus rubellus. Environmental Pollution 157 (8-9) , pp. 2459-2469. 10.1016/j.envpol.2009.03.015

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Abstract

The earthworm Lumbricus rubellus (Hoffmeister, 1843) is a terrestrial pollution sentinel. Enzyme activity and transcription of phase II detoxification superfamily glutathione transferases (GST) is known to respond in earthworms after soil toxin exposure, suggesting GST as a candidate molecular-based pollution biomarker. This study combined sub-proteomics, bioinformatics and biochemical assay to characterise the L. rubellus GST complement as pre-requisite to initialise assessment of the applicability of GST as a biomarker. L. rubellus possesses a range of GSTs related to known classes, with evidence of tissue-specific synthesis. Two affinity-purified GSTs dominating GST protein synthesis (Sigma and Pi class) were cloned, expressed and characterised for enzyme activity with various substrates. Electrospray ionisation mass spectrometry (ESI-MS) and tandem mass spectrometry (MS/MS) following SDS-PAGE were superior in retaining subunit stability relative to two-dimensional gel electrophoresis (2-DE). This study provides greater understanding of Phase II detoxification GST superfamily status of an important environmental pollution sentinel organism.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: Glutathione transferase; Lumbricus rubellus; Earthworm; 2-DE
Publisher: Elsevier
ISSN: 0269-7491
Last Modified: 24 Jun 2017 06:20
URI: http://orca.cf.ac.uk/id/eprint/8945

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