Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Identification of the pollen self-incompatibility determinant in Papaver rhoeas

Wheeler, Michael J., De Graaf, Barend H. J., Hadjiosif, Natalie, Perry, Ruth M., Poulter, Natalie S., Osman, Kim, Vatovec, Sabina, Harper, Andrea, Franklin, F. Christopher H. and Franklin-Tong, Veronica E. 2009. Identification of the pollen self-incompatibility determinant in Papaver rhoeas. Nature 459 (7249) , 992 -995. 10.1038/nature08027

[img]
Preview
PDF (Amendment 2/12/15) - Other
Download (200kB) | Preview

Abstract

Higher plants produce seed through pollination, using specific interactions between pollen and pistil. Self-incompatibility is an important mechanism used in many species to prevent inbreeding; it is controlled by a multi-allelic S locus1, 2. ‘Self’ (incompatible) pollen is discriminated from ‘non-self’ (compatible) pollen by interaction of pollen and pistil S locus components, and is subsequently inhibited. In Papaver rhoeas, the pistil S locus product is a small protein that interacts with incompatible pollen, triggering a Ca2+-dependent signalling network, resulting in pollen inhibition and programmed cell death3, 4, 5, 6, 7. Here we have cloned three alleles of a highly polymorphic pollen-expressed gene, PrpS (Papaver rhoeas pollen S), from Papaver and provide evidence that this encodes the pollen S locus determinant. PrpS is a single-copy gene linked to the pistil S gene (currently called S, but referred to hereafter as PrsS for Papaver rhoeas stigma S determinant). Sequence analysis indicates that PrsS and PrpS are equally ancient and probably co-evolved. PrpS encodes a novel ~20-kDa protein. Consistent with predictions that it is a transmembrane protein, PrpS is associated with the plasma membrane. We show that a predicted extracellular loop segment of PrpS interacts with PrsS and, using PrpS antisense oligonucleotides, we demonstrate that PrpS is involved in S-specific inhibition of incompatible pollen. Identification of PrpS represents a major advance in our understanding of the Papaver self-incompatibility system. As a novel cell–cell recognition determinant it contributes to the available information concerning the origins and evolution of cell–cell recognition systems involved in discrimination between self and non-self, which also include histocompatibility systems in primitive chordates and vertebrates.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Publisher: Nature Publishing Group
ISSN: 0028-0836
Last Modified: 04 Sep 2018 11:16
URI: http://orca.cf.ac.uk/id/eprint/8986

Citation Data

Cited 133 times in Google Scholar. View in Google Scholar

Cited 115 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics