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Night/day changes in pineal expression of >600 genes central role of adrenergic/cAMP signaling

Bailey, Michael J., Coon, Steven L., Carter, David Allan, Humphries, Ann, Kim, Jong-So, Shi, Qiong, Gaildrat, Pascaline, Morin, Fabrice, Ganguly, Surajit, Hogenesch, John B., Weller, Joan L., Rath, Martin F., Møller, Morten, Baler, Ruben, Sugden, David, Rangel, Zoila G., Munson, Peter J. and Klein, David C. 2009. Night/day changes in pineal expression of >600 genes central role of adrenergic/cAMP signaling. Journal of Biological Chemistry 284 (12) , pp. 7606-7622. 10.1074/jbc.M808394200

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Abstract

The pineal gland plays an essential role in vertebrate chronobiology by converting time into a hormonal signal, melatonin, which is always elevated at night. Here we have analyzed the rodent pineal transcriptome using Affymetrix GeneChip® technology to obtain a more complete description of pineal cell biology. The effort revealed that 604 genes (1,268 probe sets) with Entrez Gene identifiers are differentially expressed greater than 2-fold between midnight and mid-day (false discovery rate <0.20). Expression is greater at night in ∼70%. These findings were supported by the results of radiochemical in situ hybridization histology and quantitative real time-PCR studies. We also found that the regulatory mechanism controlling the night/day changes in the expression of most genes involves norepinephrine-cyclic AMP signaling. Comparison of the pineal gene expression profile with that in other tissues identified 334 genes (496 probe sets) that are expressed greater than 8-fold higher in the pineal gland relative to other tissues. Of these genes, 17% are expressed at similar levels in the retina, consistent with a common evolutionary origin of these tissues. Functional categorization of the highly expressed and/or night/day differentially expressed genes identified clusters that are markers of specialized functions, including the immune/inflammation response, melatonin synthesis, photodetection, thyroid hormone signaling, and diverse aspects of cellular signaling and cell biology. These studies produce a paradigm shift in our understanding of the 24-h dynamics of the pineal gland from one focused on melatonin synthesis to one including many cellular processes.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Publisher: American Society for Biochemistry and Molecular Biology
ISSN: 0021-9258
Last Modified: 04 Jun 2017 02:06
URI: http://orca.cf.ac.uk/id/eprint/8998

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