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Red light treatment in an axotomy model of neurodegeneration

Beirne, Kathy, Rozanowska, Malgorzata and Votruba, Marcela 2016. Red light treatment in an axotomy model of neurodegeneration. Photochemistry and Photobiology 92 (4) , pp. 624-631. 10.1111/php.12606

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Abstract

Red light has been shown to provide neuroprotective effects. Axotomising the optic nerve initiates retinal ganglion cell (RGC) degeneration, and an early marker of this is dendritic pruning. We hypothesised that 670 nm light can delay axotomy induced dendritic pruning in the retinal explant. To test this hypothesis, we monitored the effects of 670 nm light (radiant exposure of 31.7 J/cm2), on RGC dendritic pruning in retinal explants from C57BL/6J mice, at 40 minutes, 8 hours and 16 hours post axotomy. For sham-treated retinae, area under the Sholl curve, peak of the Sholl curve and dendritic length at 8 hours post axotomy showed statistically significant reductions by 42.3% (p=0.008), 29.8% (p=0.007) and 38.4% (p=0.038), respectively, which were further reduced after 16 hours by 40.56% (p<0.008), 33.9% (p<0.007), 45.43% (p<0.006), respectively. Dendritic field area was also significantly reduced after 16 hours, by 44.23% (p<0.019). Such statistically significant reductions were not seen in light-treated RGCs at 8 or 16 hours post axotomy. The results demonstrate the ability of 670 nm light to partially prevent ex vivo dendropathy in the mouse retina, suggesting that it is worth exploring as a treatment option for dendropathy associated neurodegenerative diseases, including glaucoma and Alzheimer's disease.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Publisher: Wiley-Blackwell
ISSN: 0031-8655
Funders: Fight for Sight, UK
Date of First Compliant Deposit: 13 May 2016
Date of Acceptance: 9 May 2016
Last Modified: 13 Jul 2017 00:02
URI: http://orca.cf.ac.uk/id/eprint/90851

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