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Clonal heterogeneity in the neuronal and glial differentiation of dental pulp stem/progenitor cells

Young, Fraser, Telezhkin, Vsevolod, Youde, Sarah, Langley, Martin, Stack, Maria, Kemp, Paul, Waddington, Rachel, Sloan, Alastair and Song, Bing 2016. Clonal heterogeneity in the neuronal and glial differentiation of dental pulp stem/progenitor cells. Stem Cells International , pp. 1-10. 10.1155/2016/1290561

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Abstract

Cellular heterogeneity presents an important challenge to the development of cell-based therapies where there is a fundamental requirement for predictable and reproducible outcomes. Transplanted Dental Pulp Stem/Progenitor Cells (DPSCs) have demonstrated early promise in experimental models of spinal cord injury and stroke, despite limited evidence of neuronal and glial-like differentiation after transplantation. Here, we report, for the first time, on the ability of single cell-derived clonal cultures of murine DPSCs to differentiate in vitro into immature neuronal-like and oligodendrocyte-like cells. Importantly, only DPSC clones with high nestin mRNA expression levels were found to successfully differentiate into Map2 and NF-positive neuronal-like cells. Neuronally differentiated DPSCs possessed a membrane capacitance comparable with primary cultured striatal neurons and small inward voltage-activated K+ but not outward Na+ currents were recorded suggesting a functionally immature phenotype. Similarly, only high nestin-expressing clones demonstrated the ability to adopt Olig1, Olig2, and MBP-positive immature oligodendrocyte-like phenotype. Together, these results demonstrate that appropriate markers may be used to provide an early indication of the suitability of a cell population for purposes where differentiation into a specific lineage may be beneficial and highlight that further understanding of heterogeneity within mixed cellular populations is required.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Biosciences
Dentistry
Publisher: SAGE-Hindawi Access to Research
ISSN: 1687-9678
Funders: European Research Council
Date of First Compliant Deposit: 19 May 2016
Date of Acceptance: 8 May 2016
Last Modified: 22 Feb 2019 22:21
URI: http://orca.cf.ac.uk/id/eprint/91061

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