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Developmental regulation and extracellular release of a VSG expression-site-associated gene product from Trypanosoma brucei bloodstream forms

Barnwell, E. M., van Deursen, F. J., Jeacock, L., Smith, Katherine A., Maizels, R. M., Acosta-Serrano, A. and Matthews, K. 2010. Developmental regulation and extracellular release of a VSG expression-site-associated gene product from Trypanosoma brucei bloodstream forms. Journal of Cell Science 123 (19) , pp. 3401-3411. 10.1242/jcs.068684

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Abstract

Trypanosomes evade host immunity by exchanging variant surface glycoprotein (VSG) coats. VSG genes are transcribed from telomeric expression sites, which contain a diverse family of expression-site-associated genes (ESAGs). We have discovered that the mRNAs for one ESAG family, ESAG9, are strongly developmentally regulated, being enriched in stumpy forms, a life-cycle stage in the mammalian bloodstream that is important for the maintenance of chronic parasite infections and for tsetse transmission. ESAG9 gene sequences are highly diverse in the genome and encode proteins with weak similarity to the massively diverse MASP proteins in Trypanosoma cruzi. We demonstrate that ESAG9 proteins are modified by N-glycosylation and can be shed to the external milieu, this being dependent upon coexpression with at least one other family member. The expression profile and extracellular release of ESAG9 proteins represents a novel and unexpected aspect of the transmission biology of trypanosomes in their mammalian host. We suggest that these molecules might interact with the external environment, with possible implications for infection chronicity or parasite transmission.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QR Microbiology > QR180 Immunology
Uncontrolled Keywords: Trypanosoma brucei, Antigenic variation, ESAG, Transmission, Secretion
Publisher: The Company of Biologists Ltd
ISSN: 0021-9533
Date of Acceptance: 30 June 2010
Last Modified: 18 Jan 2017 05:35
URI: http://orca.cf.ac.uk/id/eprint/91199

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