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Post-synaptic action potentials are required for nitric oxide-dependent LTP in CA1 neurons of adult GluR1 knockout and wild-type mice

Phillips, K. G., Hardingham, Neil Robert and Fox, Kevin Dyson 2008. Post-synaptic action potentials are required for nitric oxide-dependent LTP in CA1 neurons of adult GluR1 knockout and wild-type mice. Journal of Neuroscience 28 (52) , pp. 14031-14041. 10.1523/JNEUROSCI.3984-08.2008

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Abstract

Neocortical long-term potentiation (LTP) consists of both presynaptic and postsynaptic components that rely on nitric oxide (NO) and the GluR1 subunit of the AMPA receptor, respectively. In this study, we found that hippocampal LTP, induced by theta-burst stimulation in mature (>8-week-old) GluR1 knock-out mice was almost entirely NO dependent and involved both the α splice variant of NO synthase-1 and the NO synthase-3 isoforms of NO synthase. Theta-burst induced LTP was also partly NO-dependent in wild-type mice and made up ∼50% of the potentiation 2 h after tetanus. Theta-burst stimulation reliably produced postsynaptic spikes, including a high probability of complex spikes. Inhibition of postsynaptic somatic spikes with intracellular QX314 or local TTX application prevented LTP in the GluR1 knock-out mice and also blocked the NO component of LTP in wild types. We conclude that theta-burst stimulation is particularly well suited to producing the postsynaptic somatic spikes required for NO-dependent LTP.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Uncontrolled Keywords: Plasticity; potentiation; nNOS; eNOS; working memory; long-term memory
Publisher: Society for Neuroscience
ISSN: 0270-6474
Date of First Compliant Deposit: 30 March 2016
Last Modified: 03 Nov 2017 01:11
URI: http://orca.cf.ac.uk/id/eprint/9173

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Cited 21 times in Web of Science. View in Web of Science.

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