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Brain-derived neurotrophic factor prevents dendritic retraction of adult mouse retinal ganglion cells

Binley, Kate E., Ng, Wai S., Barde, Yves-Alain, Song, Bing and Morgan, James Edwards 2016. Brain-derived neurotrophic factor prevents dendritic retraction of adult mouse retinal ganglion cells. European Journal of Neuroscience 44 (3) , pp. 2028-2039. 10.1111/ejn.13295

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Abstract

We used cultured adult mouse retinae as a model system to follow and quantify the retraction of dendrites using diolistic labelling of retinal ganglion cells (RGCs) following explantation. Cell death was monitored in parallel by nuclear staining as ‘labelling’ with RGC and apoptotic markers was inconsistent and exceedingly difficult to quantify reliably. Nuclear staining allowed us to delineate a lengthy time window during which dendrite retraction can be monitored in the absence of RGC death. The addition of brain-derived neurotrophic factor (BDNF) produced a marked reduction in dendritic degeneration, even when application was delayed for 3 days after retinal explantation. These results suggest that the delayed addition of trophic factors may be functionally beneficial before the loss of cell bodies in the course of conditions such as glaucoma.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Dentistry
Optometry and Vision Sciences
Publisher: Wiley
ISSN: 0953-816X
Date of First Compliant Deposit: 22 June 2016
Date of Acceptance: 6 June 2016
Last Modified: 05 Nov 2019 04:11
URI: http://orca.cf.ac.uk/id/eprint/92056

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