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DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities

Rochelle, Paul A., Fry, John Christopher, Parkes, Ronald and Weightman, Andrew John 1992. DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. FEMS Microbiology Letters 100 (1-3) , pp. 59-65. 10.1111/j.1574-6968.1992.tb14019.x

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Abstract

A protocol was devised which permitted the extraction of DNA from deep marine sediments up to 503 m below the sea floor. These sediments have been laid down over the last 3 million years. 16S rRNA gene sequences were amplified from the DNA by the polymerase chain reaction. The details of the successful extraction and polymerase chain reaction methodology varied between samples from different depths. This emphasizes the attention to detail required to allow the diversity of bacteria in these deep sediments to be studied.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Earth and Ocean Sciences
Subjects: Q Science > QR Microbiology
Uncontrolled Keywords: DNA extraction; Polymerase chain reaction; 16S rRNA gene; Marine sediment
Publisher: Wiley-Blackwell
ISSN: 0378-1097
Last Modified: 04 Jun 2017 02:07
URI: http://orca.cf.ac.uk/id/eprint/9310

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