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FolR1: a novel cell surface marker for isolating midbrain dopamine neural progenitors and nascent dopamine neurons

Gennet, Nicole, Tamburini, Claudia, Nan, Xinsheng and Li, Meng 2016. FolR1: a novel cell surface marker for isolating midbrain dopamine neural progenitors and nascent dopamine neurons. Scientific Reports 6 , 32488. 10.1038/srep32488

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Abstract

Cell type-specific surface markers offer a powerful tool for purifying defined cell types for restorative therapies and drug screenings. Midbrain dopaminergic neurons (mesDA) are the nerve cells preferentially lost in the brains of Parkinson’s disease patients. Clinical trials of transplantation of fetal neural precursors suggest that cell therapy may offer a cure for this devastating neurological disease. Many lines of preclinical studies demonstrate that neural progenitors committed to dopaminergic fate survive and integrate better than postmitotic DA neurons. We show that the folate-receptor 1 (FolR1), a GPI-anchored cell surface molecule, specifically marks mesDA neural progenitors and immature mesDA neurons. FolR1 expression superimposes with Lmx1a, a bona-fide mesDA lineage marker, during the active phase of mesDA neurogenesis from E9.5 to E14.5 during mouse development, as well as in ESC-derived mesDA lineage. FolR1+ neural progenitors can be isolated by FACS or magnetic sorting (MAC) which give rise to dopamine neurons expressing TH and Pitx3, whilst FolR1 negative cells generate non-dopaminergic neurons and glia cells. This study identifies FolR1 as a new cell surface marker selectively expressed in mesDA progenitors in vivo and in vitro and that can be used to enrich in vitro differentiated TH neurons.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Biosciences
Medicine
Neuroscience and Mental Health Research Institute (NMHRI)
Publisher: Nature Publishing Group
ISSN: 2045-2322
Funders: MRC
Date of First Compliant Deposit: 12 September 2016
Date of Acceptance: 8 August 2016
Last Modified: 23 Feb 2019 23:54
URI: http://orca.cf.ac.uk/id/eprint/94479

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