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A partially purified acinetobacter baumannii phage preparation exhibits no cytotoxicity in 3T3 mouse fibroblast cells

Henein, Alexandra E., Hanlon, Geoffrey W., Cooper, Callum J., Denyer, Stephen P. and Maillard, Jean-Yves 2016. A partially purified acinetobacter baumannii phage preparation exhibits no cytotoxicity in 3T3 mouse fibroblast cells. Frontiers in Microbiology 7 , 1198. 10.3389/fmicb.2016.01198

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Abstract

A surge in the level and scale of antibiotic resistance has prompted renewed interest in the application of bacteriophages to treat bacterial infections. However, concerns still exist over their efficacy and safety. Acinetobacter baumannii phage BS46, a member of the family Myoviridae, has previously been shown to be effective in murine models. The cytotoxic effect of this phage was evaluated in mouse fibroblast 3T3 cells using four different assays: trypan blue; staining with Hoechst and propidium iodide; lactate dehydrogenase release; and the MTS assay. The addition of phage concentrations up to 2 × 109 pfu/mL showed little to no impact on the viability of 3T3 cells after 24 h exposure using the different assays. This study demonstrates that phage BS46 is noncytotoxic to 3T3 cells using four different assays and that appropriate quality assurance protocols for phage therapeutics are required.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Pharmacy
Publisher: Frontiers Media
ISSN: 1664-302X
Date of First Compliant Deposit: 14 October 2016
Date of Acceptance: 19 July 2016
Last Modified: 04 Jun 2017 09:27
URI: http://orca.cf.ac.uk/id/eprint/95339

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