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Generation of mouse lines with conditionally or constitutively inactivated Snca gene and Rosa26-stop-lacZ reporter located in cis on the mouse chromosome 6

Roman, Andrei Yu, Limorenko, Galina, Ustyugov, Alexey A., Tarasova, Tatiana, Lysikova, Ekaterina A., Buchman, Vladimir L. and Ninkina, Natalia 2017. Generation of mouse lines with conditionally or constitutively inactivated Snca gene and Rosa26-stop-lacZ reporter located in cis on the mouse chromosome 6. Transgenic Research 26 (2) , pp. 301-307. 10.1007/s11248-016-9995-8

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Abstract

α-Synuclein is involved in many important molecular processes in neuronal cells and their synapses, and its malfunction has been linked to the development of Parkinson’s and certain other neurodegenerative diseases. Animal models allowing tightly monitored conditional inactivation of the encoding gene, Snca, are indispensible for studies aimed at understanding normal function of α-synuclein in various neuronal populations and its role in pathogenesis of neurodegenerative diseases. We have recently reported the production of several novel mouse lines for manipulating expression of the endogenous Snca gene, including a line for Cre-recombinase-driven conditional inactivation of the gene (mice with floxed Snca) and a new line with a constitutive knockout of α-synuclein. Rosa26-stop-lacZ reporter cassette is commonly used for monitoring efficiency of Cre-recombination but in mouse genome Snca and Rosa26 loci are located on the same chromosome. Here we describe production of lines with a modified Snca locus, either floxed or constitutively inactivated and the Rosa26-stop-lacZ reporter cassette located in cis on the mouse chromosome 6. These new mouse lines are invaluable for fast identification of cells with inactivation of Snca by Cre-recombination and represent useful tools for in vivo studies of α-synuclein function and dysfunction

Item Type: Article
Date Type: Publication
Status: Published
Schools: Advanced Research Computing @ Cardiff (ARCCA)
Biosciences
Subjects: Q Science > QH Natural history > QH426 Genetics
Uncontrolled Keywords: Synuclein Conditional gene knockout Meiotic recombination Transgenic mice LoxP/Cre recombination
Publisher: Springer
ISSN: 0962-8819
Date of First Compliant Deposit: 25 January 2017
Date of Acceptance: 31 October 2016
Last Modified: 26 Dec 2018 21:55
URI: http://orca.cf.ac.uk/id/eprint/96617

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