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PLGA nano/micro particles encapsulated with pertussis toxoid (PTd) enhances Th1/Th17 immune response in a murine model

Li, Pan, Asokanathan, Catpagavalli, Lui, Fang, Khaing, Kyi Kyi, Kmiec, Dorota, Wei, Xiao-Qing, Song, Bing, Xing, Dorothy and Kong, Deling 2016. PLGA nano/micro particles encapsulated with pertussis toxoid (PTd) enhances Th1/Th17 immune response in a murine model. International Journal of Pharmaceutics 513 (1-2) , pp. 183-190. 10.1016/j.ijpharm.2016.08.059

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Abstract

Poly(lactic-co-glycolic acid) (PLGA) based nano/micro particles were investigated as a potential vaccine platform for pertussis antigen. Presentation of pertussis toxoid as nano/micro particles (NP/MP) gave similar antigen-specific IgG responses in mice compared to soluble antigen. Notably, in cell line based assays, it was found that PLGA based nano/micro particles enhanced the phagocytosis of fluorescent antigen-nano/micro particles by J774.2 murine monocyte/macrophage cells compared to soluble antigen. More importantly, when mice were immunised with the antigen-nano/micro particles they significantly increased antigen-specific Th1 cytokines INF-γ and IL-17 secretion in splenocytes after in vitro re-stimulation with heat killed Bordetalla pertussis, indicating the induction of a Th1/Th17 response. Also, presentation of pertussis antigen in a NP/MP formulation is able to provide protection against respiratory infection in a murine model. Thus, the NP/MP formulation may provide an alternative to conventional acellular vaccines to achieve a more balanced Th1/Th2 immune response.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > R Medicine (General)
R Medicine > RK Dentistry
Uncontrolled Keywords: Pertussis; PLGA nano/micro particles; Phagocytosis; Adjuvant
Publisher: Elsevier
ISSN: 0378-5173
Last Modified: 14 Oct 2017 07:07
URI: http://orca.cf.ac.uk/id/eprint/96880

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