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Interaction of an endosomolytic polyamidoamine ISA23 with vesicles mimicking intracellular membranes: A SANS/EPR study

Griffiths, Peter Charles, Nilmini, Renuka, Carter, Emma, Dodds, Patrick, Murphy, Damien Martin, Khayat, Zeena, Lattanzio, Ettore, Ferruti, Paolo, Heenan, Richard K., King, Stephen M. and Duncan, Ruth 2010. Interaction of an endosomolytic polyamidoamine ISA23 with vesicles mimicking intracellular membranes: A SANS/EPR study. Macromolecular Bioscience 10 (8) , pp. 963-973. 10.1002/mabi.201000040

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Abstract

The mechanism of ISA23 · HCl interaction with model membrane vesicles (80–100 nm in diameter) was investigated using EPR in conjunction with SANS. For EPR, 16-DSE was dissolved in the vesicle membrane to measure its dynamics and polarity, whereas a spin-labeled (Tempo)-ISA 23 analogue was used to give a measure of the polymer flexibility. When ISA23 was added to the external vesicle surface, no interaction was found. This observation conflicts with the reported ability to lyse RBC, but is in agreement with recent studies that showed no effect on membrane permeability when a PAA was added to an incubation medium containing isolated lysosomal vesicles. The vesicle-mimetic models used here provide a new and useful tool for studying endosomolytic polymer/membrane interactions.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Pharmacy
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica
Uncontrolled Keywords: Electron spin resonance ; Endosomolytic polymers ; Membranes ; Polymer/surface interactions ; Spin labels
Publisher: Wiley-Blackwell
ISSN: 1616-5187
Funders: EPSRC
Last Modified: 03 Apr 2018 20:36
URI: http://orca.cf.ac.uk/id/eprint/9746

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