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Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung

Morabito, K M, Ruckwardt, T R, Redwood, A J, Moin, S M, Price, David and Graham, B S 2017. Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung. Mucosal Immunology 10 (2) , pp. 545-554. 10.1038/mi.2016.48

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Abstract

Cytomegalovirus vectors are promising delivery vehicles for vaccine strategies that aim to elicit effector CD8+ T cells. To determine how the route of immunization affects CD8+ T cell responses in the lungs of mice vaccinated with a murine cytomegalovirus vector expressing the respiratory syncytial virus matrix (M) protein, we infected CB6F1 mice via the intranasal or intraperitoneal route and evaluated the M-specific CD8+ T cell response at early and late time points. We found that intranasal vaccination generated robust and durable tissue-resident effector and effector memory CD8+ T cell populations that were undetectable after intraperitoneal vaccination. The generation of these antigen-experienced cells by intranasal vaccination resulted in earlier T cell responses, interferon gamma secretion, and viral clearance after respiratory syncytial virus challenge. Collectively, these findings validate a novel approach to vaccination that emphasizes the route of delivery as a key determinant of immune priming at the site of vulnerability.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Additional Information: This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License
Publisher: Nature Publishing Group
ISSN: 1933-0219
Date of First Compliant Deposit: 31 January 2017
Date of Acceptance: 14 April 2016
Last Modified: 09 Jul 2019 15:55
URI: http://orca.cf.ac.uk/id/eprint/97896

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