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ShenLingLan influences the attachment and migration of ovarian cancer cells potentially through the GSK3 pathway

Owen, Sioned, Ruge, Fiona, Gao, Yunong, Yang, Ying, Hou, Jianqing, Chen, Jian, Feng, Yi, Zhang, Huiming, Wei, Cong, Wu, Yiling and Jiang, Wen Guo 2017. ShenLingLan influences the attachment and migration of ovarian cancer cells potentially through the GSK3 pathway. Medicines 7 , pp. 1-11.

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Abstract

Background: Ovarian cancer presents a major clinical challenge in the UK. Glycogen synthase kinase-3 (GSK-3) has been linked to cancer. This study tested the impact of ShenLingLan (SLDM) on ovarian cancer cell behaviour and its links to GSK-3. Methods: Fresh ovarian tumours (n = 52) were collected and processed. Histopathologcial and clinical information were collected and analysed against GSK-3 transcript levels using quantitative PCR (qPCR). Immortalised ovarian cancer cells’ protein alterations in response to SLDM were identified using a Kinexus™ protein kinase array. The effects of SLDM and a combination of SLDM and TWS119 on ovarian cancer cells ability to attach and migrate were evaluated using electrical cell-substrate impedance sensing (ECIS). Results: Transcript expression of GSK-3β was significantly increased in ovarian tumours which were poorly differentiated, patients with recurrence and in patients who had died from ovarian cancer. Treating SKOV-3 ovarian cells with SLDM reduced GSK-3 expression and GSK-3α (Y279). Treatment with SLDM reduced ovarian cancer cells ability to attach and migrate, which was further reduced in the presence of TWS119. Conclusions: This study identified a potential mechanism by which SLDM may exert anti-metastatic effects. Further work is needed to investigate the in vivo effects SLDM has on ovarian tumours.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords: ShenLingLan; ovarian cancer; cell migration; GSK3
Publisher: MDPI Publishing
Funders: Welsh National Research Network/Ser Cymru, Cancer Research Wales, the Cardiff University China Medical Scholarship
Date of First Compliant Deposit: 21 February 2017
Date of Acceptance: 15 February 2017
Last Modified: 27 Jan 2019 22:00
URI: http://orca.cf.ac.uk/id/eprint/98443

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