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Establishing species-specific sexing markers suitable for non-invasive samples of species lacking genomic resources: an example using the highly endangered common hamster Cricetus cricetus

Reiners, Tobias E, Fuchs, Melanie, Hailer, Frank, Janke, Axel and Nowak, Carsten 2017. Establishing species-specific sexing markers suitable for non-invasive samples of species lacking genomic resources: an example using the highly endangered common hamster Cricetus cricetus. Conservation Genetics Resources 9 (2) , pp. 253-255. 10.1007/s12686-016-0664-2

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Abstract

Here we present an approach to establish species-specific genetic markers for sex identification suitable for non-invasive samples. Such markers are not yet available for the endangered common hamster (Cricetus cricetus) because of the lack of genomic resources. Using Y chromosome conserved anchored tagged sequences (YCATS) exonic primers, we obtained Y-chromosomal sequences from hamsters and sympatric rodent species. From this, we designed hamster-specific primers targeting two short Y-chromosomal intron fragments and included them in microsatellite multiplex reactions, using autosomal loci also as amplification controls. The method yielded highly consistent results. The approach can be easily applied to development of sex markers in species for which there are no genome sequences available and thus aid conservation genetics efforts.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QL Zoology
Uncontrolled Keywords: Cricetus cricetus Molecular sexing Noninvasive Species-specific YCATS Y chromosome marker
Publisher: Springer Verlag
ISSN: 1877-7252
Date of First Compliant Deposit: 29 January 2018
Date of Acceptance: 5 December 2016
Last Modified: 30 Jan 2018 10:56
URI: http://orca.cf.ac.uk/id/eprint/99607

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  • Establishing species-specific sexing markers suitable for non-invasive samples of species lacking genomic resources: an example using the highly endangered common hamster Cricetus cricetus. (deposited 05 Apr 2017 12:58) [Currently Displayed]

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