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Embryonic stem cell derived neural progenitors display temporal restriction to neural patterning

Bouhon, Isabelle A., Joannides, Alexis, Kato, Hidemasa, Chandran, Siddharthan and Allen, Nicholas Denby 2006. Embryonic stem cell derived neural progenitors display temporal restriction to neural patterning. Stem Cells 24 (8) , pp. 1908-1913. 10.1634/stemcells.2006-0031

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Abstract

Neural stem cells have considerable therapeutic potential because of their ability to generate defined neuronal cell types for use in drug screening studies or cell-based therapies for neurodegenerative diseases. In this study, we differentiate mouse embryonic stem cells to neural progenitors with an initial forebrain identity in a defined system that enables systematic manipulation to generate more caudal fates, including motoneurons. We demonstrate that the ability to pattern embryonic stem cell-derived neural progenitors is temporally restricted and show that the loss of responsiveness to morphogenetic cues correlates with constitutive expression of the basic helix-loop-helix transcription factors Olig2 and Mash1, epidermal growth factor receptor, and vimentin and parallels the onset of gliogenesis. We provide evidence for two temporal classes of embryonic stem cell-derived putative radial glia that coincide with a transition from neurogenesis to gliogenesis and a concomitant loss of regional identity.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Neuroscience and Mental Health Research Institute (NMHRI)
Uncontrolled Keywords: Embryonic stem cell ; Neural differentiation ; Patterning ; Temporal restriction ; Radial glia
ISSN: 1066-5099
Last Modified: 04 Jun 2017 01:37
URI: http://orca.cf.ac.uk/id/eprint/999

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