Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing

Feng, Yi ORCID: https://orcid.org/0000-0002-2445-1290, Sanders, Andrew J. ORCID: https://orcid.org/0000-0002-7997-5286, Morgan, Liam D. ORCID: https://orcid.org/0000-0002-7571-6025, Owen, Sioned, Ruge, Fiona, Harding, Keith G and Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 2017. In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing. Scientific Reports 7 (1) , 6715. 10.1038/s41598-017-06886-6

[thumbnail of Feng Yi et al SOCS WD Scientific Reports 2017 reprint.pdf]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (2MB) | Preview
License URL: http://creativecommons.org/licenses/by/4.0
License Start date: 27 July 2017

Abstract

Wound healing and the management of chronic wounds represent a significant burden on the NHS. Members of the suppressor of cytokine signalling (SOCS) family have been implicated in the regulation of a range of cellular processes. The current study aims to explore the importance of SOCS-3 and SOCS-4 in regulating cellular traits associated with wound healing. SOCS-3 over-expression and SOCS-4 knockdown mutant lines were generated and verified using q-PCR and western blotting in human keratinocytes (HaCaT) and endothelial cells (HECV). Over-expression of SOCS-3 resulted in a significantly reduced proliferative rate in HaCaT keratinocytes and also enhanced the tubule formation capacity of HECV cells. SOCS-4 knockdown significantly reduced HaCaT migration and HECV cell tubule formation. Suppression of SOCS-4 influenced the responsiveness of HaCaT and HECV cells to EGF and TGFβ and resulted in a dysregulation of phospho-protein expression in HaCaT cells. SOCS-3 and SOCS-4 appear to play regulatory roles in a number of keratinocyte and endothelial cellular traits associated with the wound healing process and may also be able to regulate the responsiveness of these cells to EGF and TGFβ. This implies a potential regulatory role in the wound healing process and, thus highlights their potential as novel therapies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Nature Publishing Group
ISSN: 2045-2322
Funders: GlaxoSmithKline (GSK), Cancer Research Wales and Life Sciences Research Network for Wales (LSRNW)
Date of First Compliant Deposit: 27 July 2017
Date of Acceptance: 22 June 2017
Last Modified: 02 Oct 2023 05:50
URI: https://orca.cardiff.ac.uk/id/eprint/103053

Citation Data

Cited 5 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics