Multiple three-dimensional mammalian cell aggregates formed away from solid substrata in ultrasound standing waves

Kuznetsova, L. A., Bazou, D., Edwards, Gareth Owain and Coakley, William Terence 2009. Multiple three-dimensional mammalian cell aggregates formed away from solid substrata in ultrasound standing waves. Biotechnology Progress 25 (3) , pp. 834-841. 10.1002/btpr.164

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Abstract

Single and multiple three-dimensional cell aggregates of human red blood cells (RBCs) and HepG2 cells were formed rapidly in low mega-Hertz ultrasound standing wave fields of different geometries. A single discoid aggregate was formed in a half-wavelength pathlength resonator at a cell concentration sufficient to produce a 3D structure. Multiple cell aggregates were formed on the axis of a cylindrical resonator with a plane transducer (discoid aggregates); in a resonator with a tubular transducer and in the cross-fields of plane and tubular transducers and two plane orthogonal transducers (all cylindrical aggregates). Mechanically strong RBC aggregates were obtained by crosslinking with wheat germ agglutinin (WGA, a lectin). Scanning electron microscopy showed aggregate surface porous structures when RBCs were mixed with WGA before sonication and tighter packing when ultrasonically preformed aggregates were subsequently exposed to a flow containing WGA. HepG2 cell aggregates showed strong accumulation of F-actin at sites of cell-cell contact consistent with increased mechanical stability. The aggregates had a porous surface, and yet confocal microscopy revealed a tight packing of cells in the aggregate's inner core. © 2009 American Institute of Chemical Engineers Biotechnol.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Uncontrolled Keywords:	substrata; ultrasound standing waves; 3-D cell aggregates; HepG2; red blood cells
Publisher:	Wiley
ISSN:	1520-6033
Last Modified:	01 Feb 2017 03:08
URI:	https://orca.cardiff.ac.uk/id/eprint/15917

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