Nanoparticles can cause DNA damage across a cellular barrier

Bhabra, Gevdeep, Sood, Aman, Fisher, Brenton, Cartwright, Laura, Saunders, Margaret, Evans, William Howard, Surprenant, Annmarie, Lopez-Castejon, Gloria, Mann, Stephen, Davis, Sean A., Hails, Lauren A., Ingham, Eileen, Verkade, Paul, Lane, Jon, Heesom, Kate, Newson, Roger and Case, Charles Patrick 2009. Nanoparticles can cause DNA damage across a cellular barrier. Nature Nanotechnology 4 (12) , pp. 876-883. 10.1038/nnano.2009.313

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Abstract

The increasing use of nanoparticles in medicine has raised concerns over their ability to gain access to privileged sites in the body. Here, we show that cobalt–chromium nanoparticles (29.5 6.3 nm in diameter) can damage human fibroblast cells across an intact cellular barrier without having to cross the barrier. The damage is mediated by a novel mechanism involving transmission of purine nucleotides (such as ATP) and intercellular signalling within the barrier through connexin gap junctions or hemichannels and pannexin channels. The outcome, which includes DNA damage without significant cell death, is different from that observed in cells subjected to direct exposure to nanoparticles. Our results suggest the importance of indirect effects when evaluating the safety of nanoparticles. The potential damage to tissues located behind cellular barriers needs to be considered when using nanoparticles for targeting diseased states.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Nature Publishing Group
ISSN:	1748-3387
Last Modified:	17 Mar 2021 02:50
URI:	https://orca.cardiff.ac.uk/id/eprint/30314

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