Arabidopsis CULLIN3 Genes regulate primary root growth and patterning by ethylene-dependent and -independent mechanisms

Thomann, Alexis, Lechner, Esther, Hansen, Maureen, Dumbliauskas, Eva, Parmentier, Yves, Kieber, Joe, Scheres, Ben ORCID: https://orcid.org/0000-0001-5400-9578 and Genschik, Pascal 2009. Arabidopsis CULLIN3 Genes regulate primary root growth and patterning by ethylene-dependent and -independent mechanisms. Plos Genetics 5 (1) , e1000328. 10.1371/journal.pgen.1000328

Preview

PDF - Published Version Available under License Creative Commons Attribution. Download (2MB) | Preview

Abstract

CULLIN3 (CUL3) together with BTB-domain proteins form a class of Cullin-RING ubiquitin ligases (called CRL3s) that control the rapid and selective degradation of important regulatory proteins in all eukaryotes. Here, we report that in the model plant Arabidopsis thaliana, CUL3 regulates plant growth and development, not only during embryogenesis but also at post-embryonic stages. First, we show that CUL3 modulates the emission of ethylene, a gaseous plant hormone that is an important growth regulator. A CUL3 hypomorphic mutant accumulates ACS5, the rate-limiting enzyme in ethylene biosynthesis and as a consequence exhibits a constitutive ethylene response. Second, we provide evidence that CUL3 regulates primary root growth by a novel ethylene-dependant pathway. In particular, we show that CUL3 knockdown inhibits primary root growth by reducing root meristem size and cell number. This phenotype is suppressed by ethylene-insensitive or resistant mutations. Finally, we identify a function of CUL3 in distal root patterning, by a mechanism that is independent of ethylene. Thus, our work highlights that CUL3 is essential for the normal division and organisation of the root stem cell niche and columella root cap cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QK Botany
Publisher:	Public Library of Science
ISSN:	1553-7390
Date of First Compliant Deposit:	30 March 2016
Last Modified:	15 May 2023 01:54
URI:	https://orca.cardiff.ac.uk/id/eprint/57704

Citation Data

Cited 63 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item