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Minisatellite DNA markers in the chicken genome. I Distribution and abundance of minisatellites in multilocus DNA fingerprints

Bruford, Michael William ORCID: https://orcid.org/0000-0001-6357-6080 and Burke, T. 1994. Minisatellite DNA markers in the chicken genome. I Distribution and abundance of minisatellites in multilocus DNA fingerprints. Animal Genetics 25 (6) , pp. 381-389. 10.1111/j.1365-2052.1994.tb00528.x

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Abstract

This paper reports the detailed characterization of multilocus minisatellite DNA fingerprints in the chicken. Results are presented of DNA fingerprint segregation analyses carried out in three chicken pedigrees, calculating the number of detected loci, testing for Mendelian inheritance, and cosegregation among fingerprint bands. Two pedigrees (families 1 and 2) were analysed using the Jeffreys probes 33.6 and 33.15 only, and one pedigree (family 3) was analysed using 33.6, 33.15. 3′α-globin HVR and M13 protein III gene repeat. Mean band transmission frequencies in families 1 and 2 were near to the Mendelian expectation of 0.5 and no mutations were observed. Family 3 showed transmission frequencies slightly exceeding 0.5. Linkage among bands was higher than observed in some other avian species, with each allele represented by a mean of 1.48 HaeIII fragments. Cosegregation of heterozygous parental fragments representing distinguishable loci followed the expected binomial distribution. The number of minisatellites detectable by the four probes was estimated to be 217. The pattern of cosegregation among those minisatellite loci was tested against that expected for different levels of recombination through the use of a simulation model. We conclude that most minisatellites are unlinked and probably widely dispersed in the chicken genome.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Sustainable Places Research Institute (PLACES)
Publisher: Wiley-Blackwell
ISSN: 1365-2052
Last Modified: 06 Jan 2024 02:58
URI: https://orca.cardiff.ac.uk/id/eprint/65263

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