Standards for plant synthetic biology: a common syntax for exchange of DNA parts

Patron, Nicola J., Orzaez, Diego, Marillonnet, Sylvestre, Warzecha, Heribert, Matthewman, Colette, Youles, Mark, Raitskin, Oleg, Leveau, Aymeric, Farré, Gemma, Rogers, Christian, Smith, Alison, Hibberd, Julian, Webb, Alex A. R., Locke, James, Schornack, Sebastian, Ajioka, Jim, Baulcombe, David C., Zipfel, Cyril, Kamoun, Sophien, Jones, Jonathan D. G., Kuhn, Hannah, Robatzek, Silke, Van Esse, H. Peter, Sanders, Dale, Oldroyd, Giles, Martin, Cathie, Field, Rob, O'Connor, Sarah, Fox, Samantha, Wulff, Brande, Miller, Ben, Breakspear, Andy, Radhakrishnan, Guru, Delaux, Pierre-Marc, Loqué, Dominique, Granell, Antonio, Tissier, Alain, Shih, Patrick, Brutnell, Thomas P., Quick, W. Paul, Rischer, Heiko, Fraser, Paul D., Aharoni, Asaph, Raines, Christine, South, Paul F., Ané, Jean-Michel, Hamberger, Björn R., Langdale, Jane, Stougaard, Jens, Bouwmeester, Harro, Udvardi, Michael, Murray, James Augustus Henry ORCID: https://orcid.org/0000-0002-2282-3839, Ntoukakis, Vardis, Schäfer, Patrick, Denby, Katherine, Edwards, Keith J., Osbourn, Anne and Haseloff, Jim 2015. Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist 208 (1) , pp. 13-19. 10.1111/nph.13532

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Abstract

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QH Natural history > QH301 Biology
Publisher:	Wiley
ISSN:	0028-646X
Date of First Compliant Deposit:	30 March 2016
Last Modified:	04 May 2023 16:33
URI:	https://orca.cardiff.ac.uk/id/eprint/80769

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