Glycogen synthase kinase-3 is required for efficient 'Dictyostelium' chemotaxis

Teo, Regina Mei Mei, Lewis, Kimberley, Forde, Josephine E., Ryves, William Jonathan, Reddy, Jonathan Venkat, Rogers, Benjamin James and Harwood, Adrian John ORCID: https://orcid.org/0000-0003-3124-5169 2010. Glycogen synthase kinase-3 is required for efficient 'Dictyostelium' chemotaxis. Molecular Biology of the Cell 21 (15) , pp. 2788-2796. 10.1091/mbc.E09-10-0891

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Abstract

Glycogen synthase kinase-3 (GSK3) is a highly conserved protein kinase that is involved in several important cell signaling pathways and is associated with a range of medical conditions. Previous studies indicated a major role of the Dictyostelium homologue of GSK3 (gskA) in cell fate determination during morphogenesis of the fruiting body; however, transcriptomic and proteomic studies have suggested that GSK3 regulates gene expression much earlier during Dictyostelium development. To investigate a potential earlier role of GskA, we examined the effects of loss of gskA on cell aggregation. We find that cells lacking gskA exhibit poor chemotaxis toward cAMP and folate. Mutants fail to activate two important regulatory signaling pathways, mediated by phosphatidylinositol 3,4,5-trisphosphate (PIP3) and target of rapamycin complex 2 (TORC2), which in combination are required for chemotaxis and cAMP signaling. These results indicate that GskA is required during early stages of Dictyostelium development, in which it is necessary for both chemotaxis and cell signaling.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine Neuroscience and Mental Health Research Institute (NMHRI)
Subjects:	Q Science > QR Microbiology
Additional Information:	Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/1059-1524/ (accessed 24/04/2014).
Publisher:	The American Society for Cell Biology
ISSN:	1059-1524
Date of First Compliant Deposit:	30 March 2016
Last Modified:	11 Oct 2023 20:25
URI:	https://orca.cardiff.ac.uk/id/eprint/8976

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