Bacterial [methyl-3H]thymidine incorporation in substrate-amended estuarine sediment slurries

Wellsbury, Peter, Herbert, Rodney A. and Parkes, Ronald John 1994. Bacterial [methyl-3H]thymidine incorporation in substrate-amended estuarine sediment slurries. FEMS Microbiology Ecology 15 (3-4) , pp. 237-248. 10.1111/j.1574-6941.1994.tb00247.x

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Abstract

Five different bacterial communities were enriched in substrate-amended slurries of sediment from the Tay Estuary, Scotland. During incubation of the slurries, concentrations of volatile fatty acids, sulphate, sulphide and methane were monitored to clearly define the activity of the stimulated populations. An aerobic population, a ‘microaerophilic’ population and three anaerobic populations (fermentative heterotrophs, sulphate-reducing bacteria and methanogens plus acetogens) were established to reflect community growth and metabolism both in surface oxic and deeper anoxic layers. Similar numbers of cells involved in division were observed in all five slurries, demonstrating the potential for bacterial production. Thymidine incorporation rates in glucose-stimulated slurries under both aerobic and fully anaerobic conditions were similar, confirming the ability of fermentative anaerobic heterotrophs to incorporate [methyl-3H]thymidine into DNA during growth. Although anaerobic communities of sulphate-reducing, acetogenic plus methanogenic bacteria were stimulated and actively growing, they did not incorporate [methyl-3H]thymidine into DNA. Since the thymidine technique does not measure the growth of these important groups, calculated productivity values based upon thymidine incorporation within anoxic sediment systems will be substantially underestimated, even if growth substrates are not limiting.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Earth and Environmental Sciences
Subjects:	Q Science > QR Microbiology
Uncontrolled Keywords:	[methyl-3H]Thymidine incorporation; Anaerobic bacteria; Sulphate-reducing bacteria; Fermentative heterotroph; Methanogen; Stimulated slurry
Publisher:	Wiley-Blackwell
ISSN:	0168-6496
Last Modified:	04 Jun 2017 02:07
URI:	https://orca.cardiff.ac.uk/id/eprint/9307

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