Effect of desiccating stress on mouse meibomian gland function

Suhalim, Jeffrey L., Parfitt, Geraint J ORCID: https://orcid.org/0000-0002-8704-7906, Xie, Yilu, De Paiva, Cintia S., Pflugfelder, Stephen C., Shah, Tejas N., Potma, Eric O., Brown, Donald J. and Jester, James V. 2014. Effect of desiccating stress on mouse meibomian gland function. Ocular Surface 12 (1) , pp. 59-68. 10.1016/j.jtos.2013.08.002

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Abstract

Purpose Mice exposed to standardized desiccating environmental stress to induce dry eye-like symptoms have been used as a model to study the underlying mechanisms of evaporative dry eye. While studies have shown marked inflammatory and immune changes, the effect of such stress on meibomian gland function remains largely unknown. We sought to evaluate the effects of desiccating stress on meibocyte proliferation and meibum quality. Methods Ten mice were treated with scopolamine and subjected to a drafty low humidity environment (30-35%). Five and ten days after treatment, eyelids were harvested and cryosections stained with Ki67 antibody to identify cycling cells. Sections were also imaged using stimulated Raman scattering (SRS) microscopy to characterize the gland compositional changes by detecting the vibrational signatures of methylene (lipid) and amide-I (protein). Results Desiccating stress caused a 3-fold increase in basal acinar cell proliferation from 18.3 ± 11.1% in untreated mice to 64.4 ± 19.9% and 66.6 ± 13.4% after 5 and 10 days exposure, respectively (P < .001). In addition, SRS analysis showed a wider variation in the protein-to-lipid ratio throughout the gland, suggesting alterations in meibocyte differentiation and lipid synthesis. Conclusions These data are consistent with a model that a desiccating environment may have a direct effect on meibomian gland function, leading to a significant increase in basal acinar cell proliferation, abnormal meibocyte differentiation, and altered lipid production.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI)
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	evaporative dry eye; meibomian gland; nonlinear optical microscopy; stimulated Raman scattering
Publisher:	Elsevier
ISSN:	1542-0124
Date of Acceptance:	13 August 2013
Last Modified:	21 Oct 2022 07:01
URI:	https://orca.cardiff.ac.uk/id/eprint/98975

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