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MultiSite gateway-compatible cell type-specific gene-inducible system for plants

Siligato, Riccardo, Wang, Xin, Yadav, Shri Ram, Lehesranta, Satu, Ma, Guojie, Ursache, Robertas, Sevilem, Iris, Zhang, Jing, Gorte, Maartje, Prasad, Kalika, Wrzaczek, Michael, Heidstra, Renze, Murphy, Angus, Scheres, Ben and Mähönen, Ari Pekka 2016. MultiSite gateway-compatible cell type-specific gene-inducible system for plants. Plant Physiology 170 (2) , pp. 627-641. 10.1104/pp.15.01246

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Abstract

A powerful method to study gene function is expression or overexpression in an inducible, cell type-specific system followed by observation of consequent phenotypic changes and visualization of linked reporters in the target tissue. Multiple inducible gene overexpression systems have been developed for plants, but very few of these combine plant selection markers, control of expression domains, access to multiple promoters and protein fusion reporters, chemical induction, and high-throughput cloning capabilities. Here, we introduce a MultiSite Gateway-compatible inducible system for Arabidopsis (Arabidopsis thaliana) plants that provides the capability to generate such constructs in a single cloning step. The system is based on the tightly controlled, estrogen-inducible XVE system. We demonstrate that the transformants generated with this system exhibit the expected cell type-specific expression, similar to what is observed with constitutively expressed native promoters. With this new system, cloning of inducible constructs is no longer limited to a few special cases but can be used as a standard approach when gene function is studied. In addition, we present a set of entry clones consisting of histochemical and fluorescent reporter variants designed for gene and promoter expression studies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: American Society of Plant Biologists
ISSN: 0032-0889
Date of First Compliant Deposit: 6 December 2018
Date of Acceptance: 7 December 2015
Last Modified: 13 May 2019 14:26
URI: http://orca.cf.ac.uk/id/eprint/117470

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