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Establishment of a developmental compartment requires interactions between three synergistic Cis-regulatory modules

Bieli, Dimitri, Kanca, Oguz, Requena, David, Hamaratoglu Dion, Fisun, Gohl, Daryl, Schedl, Paul, Affolter, Markus, Slattery, Matthew, Mueller, Martin and Estella, Carlos 2015. Establishment of a developmental compartment requires interactions between three synergistic Cis-regulatory modules. PLoS Genetics 11 (10) , -. 10.1371/journal.pgen.1005376

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Abstract

The subdivision of cell populations in compartments is a key event during animal development. In Drosophila, the gene apterous (ap) divides the wing imaginal disc in dorsal vs ventral cell lineages and is required for wing formation. ap function as a dorsal selector gene has been extensively studied. However, the regulation of its expression during wing development is poorly understood. In this study, we analyzed ap transcriptional regulation at the endogenous locus and identified three cis-regulatory modules (CRMs) essential for wing development. Only when the three CRMs are combined, robust ap expression is obtained. In addition, we genetically and molecularly analyzed the trans-factors that regulate these CRMs. Our results propose a three-step mechanism for the cell lineage compartment expression of ap that includes initial activation, positive autoregulation and Trithorax-mediated maintenance through separable CRMs.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Public Library of Science
ISSN: 1553-7390
Date of First Compliant Deposit: 8 February 2019
Date of Acceptance: 19 June 2015
Last Modified: 08 Feb 2019 15:56
URI: http://orca.cf.ac.uk/id/eprint/118484

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