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Gompertzian growth pattern correlated with phenotypic organization of colon carcinoma, malignant glioma and non-small cell lung carcinoma cell lines

Castro, M. A. A., Klamt, F., Grieneisen, V. A., Grivicich, I. and Moreira, J. C. F. 2003. Gompertzian growth pattern correlated with phenotypic organization of colon carcinoma, malignant glioma and non-small cell lung carcinoma cell lines. Cell Proliferation 36 (2) , pp. 65-73. 10.1046/j.1365-2184.2003.00259.x

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Abstract

In the current study we present a Gompertzian model for cell growth as a function of cell phenotype using six human tumour cell lines (A‐549, NCI‐H596, NCI‐H520, HT‐29, SW‐620 and U‐251). Monolayer cells in exponential growth at various densities were quantified over a week by sulforhodamine B staining assay to produce cell‐growth curves. A Gompertz equation was fitted to experimental data to obtain, for each cell line, three empirical growth parameters (initial cell density, cell‐growth rate and carrying capacity – the maximal cell density). A cell‐shape parameter named deformation coefficient D (a morphological relationship among spreading and confluent cells) was established and compared by regression analysis with the relative growth rate parameter K described by the Gompertz equation. We have found that coefficient D is directly proportional to the growth parameter K. The fit curve significantly matches the empirical data (P < 0.05), with a correlation coefficient of 0.9152. Therefore, a transformed Gompertzian growth function was obtained accordingly to D. The degree of correlation between the Gompertzian growth parameter and the coefficient D allows a new interpretation of the growth parameter K on the basis of morphological measurements of a set of tumour cell types, supporting the idea that cell‐growth kinetics can be modulated by phenotypic organization of attached cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Wiley
ISSN: 0960-7722
Date of Acceptance: 10 January 2003
Last Modified: 26 Mar 2019 14:30
URI: http://orca.cf.ac.uk/id/eprint/120505

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