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The effects of oxygen on fermentation in Tritrichomonas foetus KV1 and its variant 1MR-100 with defective hydrogenosomes

Lloyd, David, James, Christopher J., Lloyd, Alun L., Yarlett, Nigel and Yarlett, Nuriza C. 1987. The effects of oxygen on fermentation in Tritrichomonas foetus KV1 and its variant 1MR-100 with defective hydrogenosomes. Microbiology 133 (5) , pp. 1181-1186. 10.1099/00221287-133-5-1181

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The effects of low concentrations of O2 on fermentation in the cattle parasite Tritrichomonas foetus KV1 and its variant 1MR-100 were compared using membrane inlet mass spectrometry to measure simultaneously and continuously ethanol, CO2 and H2. In strain KV1 glucose-supported H2 and CO2 production were stimulated by O2 concentrations < 1·4 μM but were inhibited at higher concentrations. Damped oscillatory responses in H2 production indicated the operation of a feedback control system. Measurement of the O2-dependence of O2 consumption rates confirmed the presence of a high-affinity terminal oxidase (apparent K m = 1·6 μM-O2 at 37 °C) and substrate inhibition by O2 at > 8 μM-O2. Successive periods of exposure to O2 resulted in decreased O2 scavenging capacity, as indicated by increasing apparent K m values for O2. The variant strain 1MR-100 which lacks pyruvate: ferredoxin oxidoreductase and hydrogenase showed quite different characteristics: H2 production was not detectable, ethanol formation was inhibited by O2 (K 1 = 1 μM) and O2-dependence of O2 consumption indicated that no high-affinity oxidase was present (apparent K m = 33 μM-O2). Progressive increases in respiration rates on repeated exposure to low O2 concentrations indicated a capacity for adaptation to aerobiosis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Microbiology Society
ISSN: 1350-0872
Last Modified: 06 Mar 2020 14:00

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