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Rapid estimation of bacterial antibiotic susceptibility with flow cytometry

MASON, D.J., ALLMAN, R., STARK, J.M. and LLOYD, D. 1994. Rapid estimation of bacterial antibiotic susceptibility with flow cytometry. Journal of Microscopy 176 (1) , pp. 8-16. 10.1111/j.1365-2818.1994.tb03494.x

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Bacterial antibiotic susceptibility was rapidly estimated for Escherichia coli and Staphylococcus spp. by flow cytometry. This was achieved by measuring the uptake of a negatively charged membrane potential sensitive dye bis‐(1,3‐dibutylbarbituric acid) trimethine oxonol and observing changes in low‐angle light scatter (excitation light scattered by up to 15°). Estimations of ampicillin, gentamicin and ciprofloxacin susceptibilities were possible within 2–5 h from a plate culture, depending on the species and antibiotic used. This includes the time necessary to establish steady‐state growth in liquid culture.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Wiley
ISSN: 0022-2720
Date of Acceptance: 9 August 1994
Last Modified: 10 Mar 2020 11:00

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