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The isolation of mitochondria from the amoeba Hartmanella castellanii Neff

Lloyd, D and Griffiths, AJ 1968. The isolation of mitochondria from the amoeba Hartmanella castellanii Neff. Experimental Cell Research 51 (2-3) , 291--300. 10.1016/0014-4827(68)90122-5

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Mitochondria were isolated from Hartmanella which satisfied various criteria of intactness and oxidized tricarboxylic acid cycle intermediates, TMPD-ascorbate and NADH. The rates of substrate oxidation were ADP dependent; P:O ratios of 2, 1.7, and 0.7 were obtained for malate, succinate and TMPD-ascorbate respectively. Antimycin A, amytal and rotenone were tested as respiratory inhibitors; the mitochondria were rotenone-insensitive. Cytochromes (a + a3), b, and c were present and changes of their redox levels were determined spectrophotometrically in various states of respiration

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0014-4827
Last Modified: 26 Feb 2020 18:56

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