Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Clonal relatedness and plasmid profiling of extensively drug-resistant New Delhi metallo-β-lactamase-producing Klebsiella pneumoniae clinical isolates

Qamar, Muhammad Usman, Ejaz, Hasan, Walsh, Timothy R., Shah, Asad Ali, Al Farraj, Dunia A., Alkufeidy, Roua M., Alkubaisi, Noorah A., Saleem, Sidrah and Jahan, Shah 2021. Clonal relatedness and plasmid profiling of extensively drug-resistant New Delhi metallo-β-lactamase-producing Klebsiella pneumoniae clinical isolates. Future Microbiology 16 (4) , 229–239. 10.2217/fmb-2020-0315

Full text not available from this repository.

Abstract

Aim: Carbapenem-resistant Klebsiella pneumoniae (CR-KP) particularly New Delhi metallo-β-lactamase (NDM) is a serious public health concern globally. The aim of the study to determine the molecular epidemiology of blaNDM-producing clinically isolated K. pneumoniae. Methods: Carbapenem-resistant K. pneumoniae isolates (n = 100) were collected from tertiary care hospital Lahore. Isolates were confirmed by VITEK® 2 system and MALDI-TOF. Minimum inhibitory concentration was performed by VITEK 2 and molecular characterization was done by PCR, PFGE, DNA hybridization and replicon typing. Results: Of 90 MBL-producing K. pneumoniae, 75 were NDM producers; 60 were NDM-1 and 11 NDM-5. A total of 27 K. pneumoniae belonged to ST11 and 14 to ST147. NDM-positive isolates were 100% resistant to β-lactam antibiotics except for colistin. 13.3% isolates carried blaNDM on ∼140 kb plasmids. A total of 32 (52.4%) isolates were positive for IncA/C and 18 (29.5%) IncF/II. Conclusion: The extensively resistant lineage of NDM-producing K. pneumoniae is prevalent in the clinical setting.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Publisher: Future Medicine
ISSN: 1746-0913
Last Modified: 12 Mar 2021 11:15
URI: http://orca.cf.ac.uk/id/eprint/139622

Actions (repository staff only)

Edit Item Edit Item