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CARS Microscopy using linearly-chirped ultrafast laser pulses

Langbein, Wolfgang, Rocha-Mendoza, Israel and Borri, Paola 2009. CARS Microscopy using linearly-chirped ultrafast laser pulses. Presented at: Lasers and Electro-Optics 2009 and the European Quantum Electronics Conference. CLEO Europe - EQEC 2009. European Conference on, Munich, Germany, 14-19 June 2009. Published in: Langbein, Wolfgang Werner, Rocha-Mendoza, Israel and Borri, Paola eds. Institute of Electrical and Electronics Engineers ( IEEE ), p. 1. 10.1109/CLEOE-EQEC.2009.5194775

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We have developed a home-built CARS microscope which exploits linearly-chirped ultrafast laser pulses to achieve spectral focussing of the vibrational excitation. CARS Stokes pulses are generated by a Ti:Sapphire laser, which pumps an optical parametric oscillator (OPO). The intra-cavity frequency-doubled signal of the OPO provides the CARS Pump. By varying the delay time between pump and Stokes their instantaneous frequency difference (IFD) changes so that CARS spectra can be measured. In fact, since CARS is generated modulating the pump by the induced coherent vibration, a pump pulse arriving after the Stokes pulse optimizes the CARS signal and reduces the non-resonant CARS background. Our microscope also features two-photon fluorescence, second harmonic generation, and differential interference contrast imaging modalities. We investigated polystyrene beads in 2% agar solution by detecting the forward-collected CARS at the C-H aromatic band.

Item Type: Conference or Workshop Item (Paper)
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Publisher: Institute of Electrical and Electronics Engineers ( IEEE )
ISBN: 9781424440795
Last Modified: 02 May 2019 11:13

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