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Applications of green fluorescent protein in cell signaling

Brennan, Paul and Astoul, E. 2001. Applications of green fluorescent protein in cell signaling. In: O'Neill, Luke A. J. and Bowie, Andrew eds. Interleukin Protocols, Vol. 60. Methods in Molecular Medicine, vol. 60. Totowa, New Jersey: Humana Press, pp. 385-391. (10.1385/1-59259-146-9:385)

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The discovery of green fluorescent protein (GFP) as a naturally fluorescing protein and its subsequent modification to allow easy detection with standard fluorescent equipment such as the fluorescence-activated cell sorter (FACS) has revolutionized our ability to detect the presence of transfected proteins and to determine their location in whole cells and in organisms. GFP was found in the bioluminescent jellyfish Aequorea (1,2). It has since been modified to change its excitation and emision (3–5). Expression vectors are now supplied commercially that allow proteins to be tagged easily (6,7). Furthermore, it is a small protein, only 29 kDa, and the DNA encoding it can be easily modified using polymerase chain reaction to introduce any restriction sites for any subcloning work required.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Humana Press
ISBN: 9780896037380
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Last Modified: 04 Jun 2017 04:04

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