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Self-assembled PAA-based nanoparticles as potential gene and protein delivery systems

Griffiths, Peter Charles, Mauro, Nicolo, Murphy, Damien Martin, Carter, Emma, Richardson, Simon C. W., Dyer, Paul and Ferruti, Paolo 2013. Self-assembled PAA-based nanoparticles as potential gene and protein delivery systems. Macromolecular Bioscience 13 (5) , pp. 641-649. 10.1002/mabi.201200462

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A series of nanoparticles is prepared via layer-by-layer assembly of oppositely charged, synthetic biocompatible polyamidoamine polymers as potential carriers. Particle size, surface charge and internal chain mobility are quantified as a function of the polymer type and number of layers. The effect of addition of surfactant is examined to simulate the effects of nanoparticle dissolution. The cyctotoxicity of these particles (in epithelia and murine cell lines) are orders of magnitude lower than polyethyleneimine controls. Stable nanoparticles may be prepared from mixtures of strongly, oppositely charged polymers, but less successfully from weakly charged polymers, and, given their acceptable toxicity characteristics, such modularly designed constructs show promise for drug and gene delivery.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH426 Genetics
Uncontrolled Keywords: gene delivery; layer-by-layer assembly; nanoparticles; self-assembly; zeta-potential
Publisher: Wiley-Blackwell
ISSN: 1616-5187
Last Modified: 13 Sep 2019 21:40

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