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ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis

Nagawa, Shingo, Xu, Tongda, Lin, Deshu, Dhonukshe, Pankaj, Zhang, Xingxing, Friml, Jiri, Scheres, Ben, Fu, Ying and Yang, Zhenbiao 2012. ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis. PLoS Biology 10 (4) , e1001299. 10.1371/journal.pbio.1001299

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Abstract

Cell polarization via asymmetrical distribution of structures or molecules is essential for diverse cellular functions and development of organisms, but how polarity is developmentally controlled has been poorly understood. In plants, the asymmetrical distribution of the PIN-FORMED (PIN) proteins involved in the cellular efflux of the quintessential phytohormone auxin plays a central role in developmental patterning, morphogenesis, and differential growth. Recently we showed that auxin promotes cell interdigitation by activating the Rho family ROP GTPases in leaf epidermal pavement cells. Here we found that auxin activation of the ROP2 signaling pathway regulates the asymmetric distribution of PIN1 by inhibiting its endocytosis. ROP2 inhibits PIN1 endocytosis via the accumulation of cortical actin microfilaments induced by the ROP2 effector protein RIC4. Our findings suggest a link between the developmental auxin signal and polar PIN1 distribution via Rho-dependent cytoskeletal reorganization and reveal the conservation of a design principle for cell polarization that is based on Rho GTPase-mediated inhibition of endocytosis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Additional Information: Full text PDG uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/1544-9173/ (accessed 18/08/2014).
Publisher: Public Library of Science
ISSN: 1544-9173
Date of First Compliant Deposit: 30 March 2016
Last Modified: 16 Jan 2019 21:10
URI: http://orca.cf.ac.uk/id/eprint/57688

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