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A plant U-box protein, PUB4, regulates asymmetric cell division and cell proliferation in the root meristem

Kinoshita, A., ten Hove, C. A., Tabata, R., Yamada, M., Shimizu, N., Ishida, T., Yamaguchi, K., Shigenobu, S., Takebayashi, Y., Iuchi, S., Kobayashi, M., Kurata, T., Wada, T., Seo, M., Hasebe, M., Blilou, I., Fukuda, H., Scheres, Ben, Heidstra, R., Kamiya, Y. and Sawa, S. 2015. A plant U-box protein, PUB4, regulates asymmetric cell division and cell proliferation in the root meristem. Development 142 (3) , pp. 444-453. 10.1242/dev.113167

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Abstract

The root meristem (RM) is a fundamental structure that is responsible for postembryonic root growth. The RM contains the quiescent center (QC), stem cells and frequently dividing meristematic cells, in which the timing and the frequency of cell division are tightly regulated. In Arabidopsis thaliana, several gain-of-function analyses have demonstrated that peptide ligands of the CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION-RELATED (CLE) family are important for maintaining RM size. Here, we demonstrate that a plant U-box E3 ubiquitin ligase, PUB4, is a novel downstream component of CLV3/CLE signaling in the RM. Mutations in PUB4 reduced the inhibitory effect of exogenous CLV3/CLE peptide on root cell proliferation and columella stem cell maintenance. Moreover, pub4 mutants grown without exogenous CLV3/CLE peptide exhibited characteristic phenotypes in the RM, such as enhanced root growth, increased number of cortex/endodermis stem cells and decreased number of columella layers. Our phenotypic and gene expression analyses indicated that PUB4 promotes expression of a cell cycle regulatory gene, CYCD6;1, and regulates formative periclinal asymmetric cell divisions in endodermis and cortex/endodermis initial daughters. These data suggest that PUB4 functions as a global regulator of cell proliferation and the timing of asymmetric cell division that are important for final root architecture.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Publisher: The Company of Biologists Ltd
ISSN: 0950-1991
Date of Acceptance: 30 November 2014
Last Modified: 08 Mar 2019 16:08
URI: http://orca.cf.ac.uk/id/eprint/71288

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